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(A) NMD of the galactose-inducible reporter transcript containing a PTC, gal-PGK1c103, was assessed in a wild-type and an edc3Δ pat1Δ double deletion strain over the course of one hour. (B and C) Steady-state pre-mRNA/mRNA ratios of the endogenous NMD transcript, CYH2, were assessed in wild-type, pat1Δ, edc3Δ pat1Δ, edc3Δ, edc1Δ, edc2Δ, edc1Δ edc2Δ, edc1Δ edc2Δ edc3Δ, edc1Δ edc3Δ, and edc2Δ edc3Δ strains.
