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. 2011 Oct 31;6(10):e26547. doi: 10.1371/journal.pone.0026547

Figure 4. Edc3-Upf1 interaction does not promote NMD.

Figure 4

Upf1 mutants that disrupt the Upf1-Edc3 interaction (mutants 3, 18, 23, and 24) or the Upf1-Edc3 and Upf1-Upf2 interaction (mutant 27) were assessed for their role in NMD. (A) Decay of a reporter transcript containing a premature termination codon (PTC) at codon 142 under a galactose-inducible promoter, gal-PGK1c142, was assessed in a upf1Δ strain containing either empty vector, Upf1 wild-type, Upf1 mutant 3, Upf1 mutant 18, Upf1 mutant 23, Upf1 mutant 24, or Upf1 mutant 27 on a plasmid. (B) Steady-state pre-mRNA/mRNA ratios of the endogenous NMD transcript, CYH2, were assessed in a upf1Δ strain containing either Upf1 (WT), empty vector, Upf1 mutant 3, Upf1 mutant 18, Upf1 mutant 23, Upf1 mutant 24, or Upf1 mutant 27 on a plasmid.