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. 2011 Oct 31;6(10):e26547. doi: 10.1371/journal.pone.0026547

Figure 5. The Edc3-Upf1 interaction is not a negative regulator of NMD.

Figure 5

(A) NMD of the PTC-containing galactose-inducible reporter transcript, gal-PGK1c142, was assessed in a upf1Δ upf2Δ double deletion strain containing either empty vector, Upf1 wild-type, Upf1 mutant 3, Upf1 mutant 18, Upf1 mutant 23, Upf1 mutant 24, or Upf1 mutant 27 on a plasmid. (B) Steady-state pre-mRNA/mRNA ratios of the endogenous NMD transcript, CYH2, were assessed in the upf1Δ upf2Δ strain containing either wild-type Upf1, empty vector, Upf1 mutant 3, Upf1 mutant 18, Upf1 mutant 23, Upf1 mutant 24, or Upf1 mutant 27 on a plasmid. (C) Steady-state pre-mRNA/mRNA ratios of CYH2 were assessed in a upf1Δ upf2Δ strain containing either wild-type Upf1 or Upf1 mutant 27 and either Upf2 mutant 13, Upf2 mutant 6-NE, Upf2 mutant 6-G, Upf2 mutant 5, empty vector, or Upf2 wild-type plasmids. (D) Steady-state pre-mRNA/mRNA ratios of CYH2 were assessed in a upf2Δ and an edc3Δ upf2Δ strain containing either Upf2 mutant 13, Upf2 mutant 6-NE, Upf2 mutant 6-G, Upf2 mutant 5, empty vector, or Upf2 wild-type plasmids.