Table 1. Specific primers used in PCR analysis.
Molecule | Primer | Sequence |
β-actin | Forward | 5′-GCTGTCCCTGTATGCCTCT-3′ |
Reverse | 5′-GTCACGCACGATTTCCCTC-3′ | |
IL-13 | Forward | 5′-GCTTATTGAGGAGCTGAG-3′ |
Reverse | 5′-GGGCTACTTCGATTTTGG-3′ | |
IL-4 | Forward | 5′-TTTGAACGAGGTCACAGG-3′ |
Reverse | 5′-GCATGATGCTCTTTAGGC-3′ | |
IL-6 | Forward | 5′-TTCTTGGGACTGATGCTG-3′ |
Reverse | 5′-CTGGCTTTGTCTTTCTTGTT-3′ | |
IL-17a | Forward | 5′-CTCAACCGTTCCACGTCAC-3′ |
Reverse | 5′-ACACCCACCAGCATCTTCT-3′ | |
IFN-γ | Forward | 5′-TCTGAGACAATGAACGCTAC-3′ |
Reverse | 5′-TGGACCACTCGGATGAG-3′ | |
iNOS | Forward | 5′-GGACGAGACGGATAGGCA-3′ |
Reverse | 5′-CATCTCGGGTGCGGTAG-3′ | |
RORα | Forward | 5′-CGCTCGTGGCTTCAGGAAAAGGT-3′ |
Reverse | 5′-AGAAGTGCTCGGGCGCGACAT-3′ | |
RORγt | Forward | 5′-CATCTCTGCAAGACTCATCG-3′ |
Reverse | 5′-CAGGGGATTCAACATCAGTG-3′ | |
TNFα | Forward | 5′-TCCCTTTCACTCACTGGC-3′ |
Reverse | 5′-ACTTGGTGGTTTGCTACG-3′ | |
Foxp3 | Forward | 5′-AGGAGAAAGCGGATACC-3′ |
Reverse | 5′-CAGGGAGGAGTTCAGTAGAG-3′ |