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. 2011 Oct 31;6(10):e27034. doi: 10.1371/journal.pone.0027034

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A) Cardiac lysates from wt and mdx animals were immunoprecipitated with an anti Dys427 antibody and probed for the presence of Kir6.2 (lower part). The membrane was then re-probed with the anti Dys427 antibody (upper part). Kir6.2 is abundant in the total lysate of both wt and mdx hearts but co-immunoprecipitates with Dys427 only in the wt sample. B) CKm interacts with dystrophin. Samples as in A were probed with an anti CKm antibody (lower panel) The membrane was subsequently reprobed with an anti Dys427 antibody (upper part). Note that CKm is abundant in the total lysate of both wt and mdx hearts but co-immuno precipitate with Dys427 only in the wt sample.