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. 2011 Oct 31;6(10):e26992. doi: 10.1371/journal.pone.0026992

Figure 5. qRT-PCR of Hspa12a and Hyou1 in different brain regions.

Figure 5

RNA was isolated from the cerebrum or brain stem of wild type (WT) and mutated (mut) mice at P3 and P21 followed by qRT-PCR analysis using primers specific for Hspa12a (A) and Hyou1 (B). Each mRNA expression level shown is the average of 3 biological samples calculated relative to GAPDH control. Data are presented as ΔCt (gene of interest/GAPDH). High ΔCt value indicates low mRNA level. The calculated relative quantity (RQ) in mut relative to WT is shown for each case; * indicates p<0.05.