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. 2011 Oct 31;6(10):e27076. doi: 10.1371/journal.pone.0027076

Figure 2. Limit of detection for the newly developed S. wadsworthensis-specific nested PCR assay.

Figure 2

The PCR assay was able to detect 0.5 pg of S. wadsworthensis DNA. Lane 1: 100 bp marker, Lane 2: negative control (no DNA), Lane 3: S. wadsworthensis DNA 50 ng/µl, Lane 4: S. wadsworthensis DNA 5 ng/µl, Lane 5: S. wadsworthensis DNA 0.5 ng/µl, Lane 6: S. wadsworthensis DNA 50 pg/µl, Lane 7:S. wadsworthensis DNA 5 pg/µl, Lane 8: S. wadsworthensis DNA 0.5 pg/µl, Lane 9: S. wadsworthensis DNA 0.05 pg/µl, Lane 10: S. wadsworthensis DNA 0.005 pg/µl.