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. 2011 Oct 31;6(10):e27230. doi: 10.1371/journal.pone.0027230

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Näsström et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Binding of mAb49/G to α-synuclein monomers (□) or α-synuclein oligomers (•) was analyzed on HNE stabilized α-synuclein oligomer coated plates. On the x-axis, the molar concentration of α-synuclein is displayed. The IC50 values are calculated as the concentration of either α-synuclein monomers or α-synuclein oligomers needed to quench half of the signal in the ELISA. Note that, due to uncertainties concerning the size of the α-synuclein oligomers used in this assay, the concentration in pM for both species is based on the molecular weight of one α-synuclein monomer. These data are representative of at least three independent experiments.