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. 2011 Oct 31;6(10):e27230. doi: 10.1371/journal.pone.0027230

Figure 4. Alpha-synuclein dimerization/oligomerization, as shown by GFP fluorescence reconstitution.

Figure 4

Forty eight hours after double transfection with the two α-synuclein hemi:GFP constructs, the H4 cells exhibited robust GFP fluorescence (2.7-fold over expression to vector controls) throughout the cell soma and nucleus (A and G). When cells were treated (+mAb) with the α-synuclein C-terminal specific antibodies mAb49/G and mAb211 the GFP fluorescence was significantly (*p<0.05, **p<0.01) reduced (1.4- and 1.5-fold over expression to vector controls respectively) indicating less dimerization/oligomerization (Fig. D, E and G). The mAb5C2 antibody targeting the non-Aβ component (NAC) region of α-synuclein did not show any reduction (2.5-fold over expression to vector controls) of GFP fluorescence, indicating no effect on the formation of dimers/oligomers (Fig. F and G). With the monoclonal antibody mAb9484 against GAPDH, no apparent effect (2.6-fold over expression to vector controls) on dimerization/oligomerization could be seen (Fig. C and G). 20X magnification. Scale bar 20 µm.