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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Neurochem. 2011 Oct 11;119(4):879–889. doi: 10.1111/j.1471-4159.2011.07485.x

Figure 4. Binge drinking of ethanol increases Fyn activation in the DMS of Long Evans rats.

Figure 4

Rats underwent ethanol drinking procedue as described in Fig. 3, thirty minutes after the initiation of the last drinking session, DMS tissue was dissected and Fyn total was IP-ed from homogenates with mouse monoclonal anti-Fyn antibodies, and anti-mouse IgG antibodies as a control. Active Fyn was detected by the anti-pY420/418[Fyn/Src] antibodies (1:500) (a). In a separate membrane, the anti-pY531/529 [Fyn/Src] antibodies (1:1000) detected inactive Fyn kinase (b). Both membranes were stripped and re-probed with polyclonal anti-Fyn antibodies to measure the level of IP-ed Fyn. Images are representative of n=3 (water), n=3 (ethanol). Bar graphs summarize the averaged effect of ethanol treatment on the levels of active and inactive Fyn by quantification of the level of pY420/418 or pY531/529 to total IP-ed Fyn. Data are expressed as percentage of water control, and are expressed as mean ± SEM. **P < 0.01, *P < 0.05 vs. water-drinking rats (two-tailed Student’s t-test).