Table 3.
Retention parameters for FITC-BSA-loaded particles obtained after fitting in vitrodrug release data (0.5–24 hours) to three different mathematical models for FITC-BSA retention kinetics
| Formulation | Mathematical modelsa for retention kinetics | ||
|---|---|---|---|
| Zero order | First order | Higuchi model | |
| Before lyophilization | |||
| FITC-BSA-liposome | k′0 = −0.0319 | k′1 = −0.0556 | k′h = −0.1953 |
| R2 = 0.7838 | R2 = 0.6688 | R2 = 0.9307 | |
| FITC-BSA lipoparticle | k′0 = −0.0314 | k′1 = −0.0882 | k′h = −0.1879 |
| R2 = 0.8543 | R2 = 0.5940 | R2 = 0.9669 | |
| After lyophilization | |||
| FITC-BSA-liposome | k′0 = −0.0377 | k′1 = −0.0735 | k′h = −0.2332 |
| R2 = 0.7580 | R2 = 0.5584 | R2 = 0.9154 | |
| FITC-BSA lipoparticle | k′0 = −0.0374 | k′1 = −0.0965 | k′h = −0.2172 |
| R2 = 0.9306 | R2 = 0.7183 | R2 = 0.9922 | |
a
Notes: Zero order, Mt/M⊥ = k′0t; first order, Mt/M⊥ = 1 − exp(−k′1 t); higuchi model, Mt/M⊥ = k′h t1/2, where Mt/M⊥ is the fraction of the drug retained at time t and k′0, k′1, and k′h, represent the zero-order release constant, first-order retention constant, and Higuchi constant, respectively.
Abbreviation: FITC-BSA, fluorescein isothiocyanate-conjugated bovine serum albumin.