. Author manuscript; available in PMC: 2011 Nov 1.

Published in final edited form as: Anal Bioanal Chem. 2011 Sep 20;401(9):2793–2803. doi: 10.1007/s00216-011-5367-7

Table 5.

SAX-HPLC analysis of 7 commercial heparin samples digested with Hep I, II or III.

Heparin Sample #
Heparin lyase 1	1	2	3	5	6	7	8	Average	Std. dev.^b
Heparin lyase 1	%^a	%	%	%	%	%	%	%	Std. dev.^b
2a^c	0.87	0.86	0.75	0.78	0.77	0.81	0.79	0.80	0.05
2b	1.22	1.01	1.18	0.99	1.05	1.08	1.15	1.10	0.09
2c	4.44	4.65	4.51	4.55	3.99	4.11	3.98	4.32	030
2d	80.40	79.7	79.08	80.07	80.89	80.58	80.85	80.22	0.70
Heparin Sample #
Heparin lyase 2	1	2	3	5	6	7	8	Average	Std. dev.
Heparin lyase 2	%	%	%	%	%	%	%	%	Std. dev.
2a	0.93	0.89	0.97	0.98	0.95	1.10	1.02	0.98	0.07
2b	15.5	14.50	15.30	14.90	15.20	15.10	13.50	14.86	0.70
2c	9.10	9.00	10.10	8.90	8.10	7.90	7.40	8.64	0.90
2d	61.60	62.02	60.54	63.24	62.29	64.13	65.97	62.83	1.8
2e	3.51	3.31	3.49	3.41	3.11	2.99	2.89	3.24	0.30
2f	3.11	2.99	3.08	3.02	3.01	2.70	3.09	3.00	0.10
2g	1.44	1.69	1.72	1.71	1.70	2.10	1.79	1.74	0.20
2h	1.41	1.40	1.45	1.39	1.42	1.51	1.40	1.43	0.04
Heparin Sample #
Heparin lyase 3	1	2	3	5	6	7	8	Average	Std. dev.
Heparin lyase 3	%	%	%	%	%	%	%	%	Std. dev.
2a	43.19	42.69	43.46	49.78	43.50	52.05	39.76	44.92	4.3
2b	4.66	4.28	3.85	4.06	5.70	4.30	4.75	4.51	0.60
2e	12.76	12.73	11.99	11.60	12.25	11.77	14.25	12.48	0.90
2f	11.61	11.2	12.1	13.92	12.75	13.91	15.20	12.96	1.5

Tetrasaccharide and higher oligosaccharide products were not quantified so that the mole % of disaccharides did not sum to 100%. Quantitative analysis of the disaccharide products (2a–2h) was performed in triplicate.

Analysis was performed on each chromatogram and the average value of each disaccharide (2a–2h) in the 7 heparins treated with Hep I, II or III was calculated and the standard deviations across the 7 heparin samples were calculated.

The structures determined for each peak are: 2a ΔUA-GlcNAc, 2b ΔUA-GlcNS6S, 2c ΔUA2S-GlcNS, 2d ΔUA2S-GlcNS6S, 2e ΔUA-GlcNS, 2f ΔUA-GlcNAc6S, 2g ΔUA2S-GlcNAc, 2h ΔUA2S-GlcNAc6S