Table 6.
Percent substitution of glucosamine (A) and uronic acids (iduronic acid I or glucuronic acid G) in heparin samples #1–8.
| Heparin Sample # | ANS % |
ANAc % |
A3S % |
A6Sa % |
I2S % |
I % |
G % |
Impurities observed |
|---|---|---|---|---|---|---|---|---|
| 1 | 81 | 13 | 6.2 | 83.4 | 61.3 | 11.5 | 27.2 | ethanol |
| 2 | 80 | 13 | 7.9 | 83.4 | 60.8 | 10.0 | 29.1 | acetate |
| 3 | 78 | 14 | 7.8 | 82.0 | 61.8 | 9.5 | 28.6 | ethanol |
| 4b | 80 | 14 | 6.6 | 87.2 | 57.0 | 13.5 | 29.5 | ethanol |
| 5 | 80 | 14 | 6.0 | 83.7 | 62.6 | 10.3 | 27.0 | acetate |
| 6 | 81 | 13 | 6.3 | 84.1 | 54.2 | 11.9 | 33.9 | acetate |
| 7 | 82 | 13 | 5.8 | 82.9 | 65.3 | 9.9 | 24.8 | acetate |
| 8 | 80 | 13 | 6.9 | 84.1 | 60.9 | 10.7 | 28.4 | ethanol |
| Avg. | 80 | 13 | 6.7 | 83.4 | 61 | 10.5 | 28.4 | ethanol |
| Std. dev. | 0.9 | 0.5 | 0.7 | 0.6 | 2.6 | 0.7 | 2.0 | |
| Var. | 0.7 | 0.2 | 0.4 | 0.3 | 5.8 | 0.4 | 3.4 |
a
A6S values obtained by the integration of the 13C spectra.
b
Sample # 4 is LMWH.