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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Arthritis Rheum. 2011 Nov;63(11):3274–3283. doi: 10.1002/art.30573

FIGURE 4. AMG487 reduces production of active MMP-1 and CXCR3-mediated calcium influx into DA and RA FLS.

FIGURE 4

(A) Densitometry analyses of MMP-1 western blot (below) of four DA FLS cell lines (mean of active/pro-MMP-1). (*P=0.02, t-test; mean±S.E.M.). (B) Western blot showing pro- and active MMP-1 (n=4). (C) CXCL10-containing 10% FBS induced a significant increase in intracellular calcium influx. Pre-treatment of FLS with AMG487 (10μM) reduced calcium influx by nearly 64% in DA FLS, (D) and by 100% in RA FLS (AMG487 100 μM), compared with FLS treated with 10% FBS only, confirming inhibition of the CXCR3 receptor signaling.