Figure 10.
MPs degrade bound matrix molecules with MMPs. (A) Fn-FITC samples co-isolated with MP or Sup were incubated for 0 or 3 days and analyzed with fluorescent scans of SDS-PAGE protein gels and integrin αV western blots. Fn-FITC incubated with MPs was degraded. (B) Samples of Fn-FITC co-precipitated with MPs and incubated with GM6001 (20 μM) exhibited less degradation than samples incubated with vehicle controls (VC). (C) EDTA (10 mM) and RGD (2.5 mM), but not RAD (2.5 mM), interfered with degradation of Fn-FITC by MPs.