Skip to main content
. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: J Cell Physiol. 2012 Feb;227(2):534–549. doi: 10.1002/jcp.22744

Figure 9.

Figure 9

MPs bind and activate exogenous MMP-2. (A) MMP-2 western blot analysis of MP fractions pre-incubated with or without MSC-CM. Ultracentrifugation pellets (UCP) collected from samples of MSC-CM incubated without MPs were included as negative controls, and blots of actin and GAPDH served as loading controls. MSC-secreted MMP-2 localized to MP fractions. (B) UCP and MPs incubated with or without MSC-CM were analyzed via MMP activity assays. *** p < 0.001 (C) MSC-CM samples were incubated with MP or Sup fractions for 0 or 20 hours and analyzed via MMP-2 western blots. Active MMP-2 was detected primarily in MSC-CM samples incubated with MPs. (D) MSC-CM samples incubated with MPs exhibited higher increases in MMP activity than those incubated with Sup fractions. *** p < 0.001 (E) Time-dependence of MMP-2 activation in MSC-CM samples incubated with MP fractions over 20 hours. Results are means ± SD.