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. Author manuscript; available in PMC: 2012 Dec 30.
Published in final edited form as: Immunol Lett. 2011 Aug 30;141(1):102–108. doi: 10.1016/j.imlet.2011.08.002

Figure 2. TLR4 and TLR7 agonists stimulate histamine production by neutrophils.

Figure 2

(A) Neutrophils were isolated from the bone marrow and stimulated in vitro (1 × 105/well) for 8 hours with various TLR agonists and histamine measured in the supernatant. The dose responsiveness production of histamine to (B) LPS and (C) R837 was then determined. Data represents 3–6 independent wells of neutrophils pooled from 5–10 mice from one (A) or three (B and C) independent experiments *=p<0.05 and ***=p<0.005 by Students t-test.