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. Author manuscript; available in PMC: 2012 Dec 30.
Published in final edited form as: Immunol Lett. 2011 Aug 30;141(1):102–108. doi: 10.1016/j.imlet.2011.08.002

Figure 3. LPS promotes release of histamine and not storage.

Figure 3

Bone marrow isolated neutrophils (1 × 105/well) were stimulated in vitro for the indicated times with LPS (100ng/ml) + GM-CSF (10ng/ml). At each time point, (A) supernatant or (B) total well content histamine levels (cell pellet plus supernatant) were determined. Data represents 3–6 independent wells of neutrophils pooled from 5–10 mice from two independent experiments.