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. Author manuscript; available in PMC: 2012 Dec 30.
Published in final edited form as: Immunol Lett. 2011 Aug 30;141(1):102–108. doi: 10.1016/j.imlet.2011.08.002

Figure 5. Inhibition of PI3-kinase prevents LPS-induced histamine production.

Figure 5

Bone marrow isolated neutrophils (1 × 105/well) were pretreated with the indicated concentrations of Ly294002 for 30 minutes prior to in vitro stimulation for 8 hours with LPS (100ng/ml) + GM-CSF (10ng/ml) and histamine production measured in the supernatant. Data represents 3–6 independent wells of neutrophils pooled from 5–10 mice from 2 independent experiments **=p<0.01, ***=p<0.005 by Students t-test.