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. Author manuscript; available in PMC: 2012 Oct 28.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Sep 28;414(3):539–544. doi: 10.1016/j.bbrc.2011.09.108

Fig. 2. Expression of cell markers in cultured P0 Ebf+/+and Ebf1−/− retinal explants.

Fig. 2

(A–N) P0 Ebf+/+and Ebf1−/− retinal explants were cultured in vitro for 8 days and their sections were then immunolabeled with the indicated antibodies. (A, B) Anti-Pax6 stains amacrine, horizontal cells and RGCs. (C, D) Anti-GLYT1 stains glycinergic amacrine cells. (E, F) Anti-GABA stains GABAergic amacrine cells. (G, H) Anti-calbindin stains horizontal and some amacrine cells. (I, J) Anti-Bhlhb5 stains type 2 OFF-cone bipolar cells and GABAergic amacrine cells. (K, L) Anti-Chx10 stains bipolar cells. (M, N) Anti-glutamine synthetase (G.S.) stains Müller cells. There are no obvious changes in these markers except the increase of G.S.-immunoreactive Müller cells labeled by G.S. GCL, ganglion cell layer; INL, inner nuclear layer; ONL, outer nuclear layer. Scale bar: J (for A–N), 50μm.