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. Author manuscript; available in PMC: 2011 Dec 1.

Published in final edited form as: Growth Factors. 2011 Sep 13;29(6):278–289. doi: 10.3109/08977194.2011.616200

INSR phosphorylation study using the PathScan^® phospho-INSR (Y1150/Y1151) ELISA kit. Figures 5A–B show a bar graph representation of INSR phosphorylation measured as OD 450 for all samples per group (AAN= 23, AAM=27, CAN=20 and CAM=24). Asterisks indicate values statistically significant (*p<0.05). Figure 5C shows representative western blot analyses of total and phosphorylated INSR, IRS-1 and FAK proteins. Figures 5D–G show bar graphs of phosphorylated IRS-1 and FAK normalized to total IRS-1 and FAK proteins in pre- and post-menopausal breast tissue samples, and presented as the mean ± SE of all samples per group. Asterisks indicate values statistically significant (*p< 0.05).

INSR phosphorylation study using the PathScan^® phospho-INSR (Y1150/Y1151) ELISA kit. Figures 5A–B show a bar graph representation of INSR phosphorylation measured as OD 450 for all samples per group (AAN= 23, AAM=27, CAN=20 and CAM=24). Asterisks indicate values statistically significant (*p<0.05). Figure 5C shows representative western blot analyses of total and phosphorylated INSR, IRS-1 and FAK proteins. Figures 5D–G show bar graphs of phosphorylated IRS-1 and FAK normalized to total IRS-1 and FAK proteins in pre- and post-menopausal breast tissue samples, and presented as the mean ± SE of all samples per group. Asterisks indicate values statistically significant (*p< 0.05).