Figure 6.
Immunohistochemistry of INSRs in paired human normal and malignant breast tissue samples. A comparison of the INSRs expression was assessed by utilizing an antibody that recognizes both receptors INSRA & INSRB (INSR α-subunit; (5D9) and a polyclonal antibody that only recognizes INSRB (see materials section). Malignant AA and CA samples correspond to ER (+) invasive ductal carcinomas (IDC) Bloom and Richardson’s grade III. Panels A–B corresponds to INSR immunostaining from AAN samples (pre- and post-menopausal). Panels E–H corresponds to INSR immunostaining from AAM samples. Panels I–L and M–P correspond to INSR immunostaining, in CAN and CAM samples respectively. Original magnifications 20X. Total number of patients (n) analyzed per group was as follows: AAN= 15, AAM=15, CAN=13 and CAM=13.