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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Biochem Pharmacol. 2011 Sep 6;82(11):1771–1780. doi: 10.1016/j.bcp.2011.08.023

Fig. 4. The effect of metformin on SHP mRNA and protein expression in primary human hepatocytes and cell lines.

Fig. 4

Primary human hepatocyte cultures, HEP220466 and HEP220492 (A, B, C), or LS174T and MZ-Hep1 cell lines (D) were treated with metformin (0.1, 0.5, 1.0 and 2.0 mM) for 24 h or 60 h. Total RNA was isolated, and SHP mRNA levels were analyzed by qRT-PCR (A,B,D). SHP protein levels were analyzed by Western blotting (C). The effect of metformin on SHP mRNA levels is presented as the relative SHP mRNA expression level normalized to HPRT housekeeping mRNA relative to control vehicle (DMSO)-treated cells (set to 1). Negative (−, untreated MZ-Hep1 cells) and positive (+, SHP -transfected MZ-Hep1 cells) controls were used for Western blotting analyses. *p<0.05; **p<0.01 indicates a statistically significant result compared to vehicle-treated controls (ANOVA with Dunnett’s posthoc test).