Table 1.

Primer sequences used for generation of GC1 subdomains. Sense and antisense primers were flanked by two unique restriction sites (third column) suitable for cloning into the XOP-0.8EGFP-C1 vector.

Construct	Sense primer	Antisense primer	Sites
GCct1	5′-ATGAGCACTGTTCGGATTC	5′-CCAGTTTACTGGGAAGTGA	BamHI;HpaI
GCct2	5′-GTGGCTGAGGCCCTGAAGA	5′-CCAGTTTACTGGGAAGTGA	EcoRI;AatII
GCct3	5′-ATGCTGGAGCAGTACTCT	5′-CCAGTTTACTGGGAAGTGA	EcoRI;AatII
GCct4	5′-TACTTGAGGCACAGGCTGC	5′-CCAGTTTACTGGGAAGTGA	EcoRI;AatII
GCct5	5′-TACTTGAGGCACAGGCTGC	5′-ATCGACTCCATGCTTCGGTAA	EcoRI;AatII
GCc6	5′-ATGCTGGAGCAGTACTCTC	5′-CAGATGCTGCCTCCATCTTAA	EcoRI;AatII
GCct7	5′-TACTTGAGGCACAGGCTGC	5′-CAGATGCTGCCTCCATCTTAA	EcoRI;AatII
GCct8	5′-ATGGACCTCACCTTTGACCT	5′-CCAGTTTACTGGGAAGTGA	EcoRI;AatII
GCtm1	5′-ATGAGCGCTTGGCTCCTGCC	5′-CAGGCCAGTTTACTGGGAAG	NheI
GCtm2	5′-ATGAGCGCTTGGCTCCTGCC	5′-GGCACAGGCTGCTACACATG	NheI
GCtm3	5′-GTGATCTGCAACG	5′-TGGGAAGTGA	BglII; EcoRI
GCtm4	5′-ATGAGCGCTTGGCTCCTGCCAG	5′-GGCCAGTTTACTGGGAAGACGGA-GACCAGCCAGGTGGCTCCAGCCTGA	BglII; EcoRI