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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cell Signal. 2011 Sep 17;24(1):265–271. doi: 10.1016/j.cellsig.2011.09.009

Figure 2.

Figure 2

The effect of PI3K/Akt signaling in 5-HT1A receptor-regulated GSK3 in the hippocampus. (A) Mice were treated as described in Figure 1. Representative immunoblots and quantification of phospho-Thr308-Akt, phospho-Ser473-Akt and total Akt (left panel). Data is expressed as % Control (saline-treated). Mean ± SEM, n=12-16, *p<0.05 in Student’s t-test when 8-OH-DPAT treatment is compared to saline treatment. Immunohistochemical images of total Akt and phospho-Thr308-Akt (right panel). Red, Akt or phospho-Thr308-Akt; Blue, Hoescht 33342 nuclear marker. (B) Intrahippocampal (ih) infusion of LY294002 (LY, 2.5 nmole/side, 90 min) followed by 8-OH-DPAT (DPAT, 1 mg/kg, i.p., 30 min). Representative immunoblots and quantified data of phospho-Thr308-Akt, total Akt, phospho-Ser9-GSK3β, and total GSK3β. Data is expressed as % Control (saline-treated). Mean ± SEM, n=4-6, *p<0.05 in one-way ANOVA followed by Holm-Sidak comparison.