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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cell Signal. 2011 Sep 17;24(1):265–271. doi: 10.1016/j.cellsig.2011.09.009

Figure 4.

Figure 4

The effect of fluoxetine in regulation of GSK3 and in FST. (A) WT mice were treated with saline (S or Sal) or fluoxetine (F or FLX, 20 mg/kg, i.p.) for 30 min, Representative immunoblots and quantification of phospho-Ser21-GSK3α, phospho-Ser9-GSK3β, total GSK3β and total GSK3β of serine-phosphorylated GSK3α and GSK3β in the hippocampus. Data is expressed as % Control (saline-treated). Mean ± SEM, n=11-14, *p<0.05 in Student’s t-test when fluoxetine is compared to saline treatment. (B) WT, S21A-GSK3α KI (αKI), S9A-GSK3β KI (βKI), and S21/9A-GSK3α/β double-KI (α/βKI) mice were treated with saline or fluoxetine (20 mg/kg, i.p.) for 30 min, followed by testing immobility in the FST. Mean ± SEM, n=6-10, *p<0.05 between saline and fluoxetine in the same genotype by Holm-Sidak comparison in two-way ANOVA. This test found no interaction between the four genotypes and the two treatments (saline and fluoxetine) (DF=3, F-1.954, p=0.131).