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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Jun 13;256(3):324–329. doi: 10.1016/j.taap.2011.05.018

Figure 3. In vitro effects chlorpyrifos oxon and WIN 55,212-2 on hippocampal and striatal ACh release in slices from wild type (WT) and CB1 knockout (KO) mice.

Figure 3

Hippocampal (A) and striatal (B) slices were incubated with [3H]choline to label endogenous acetylcholine. Prelabelled slices were then loaded into a suprafusion apparatus and perfused with physiological buffer. Release was stimulated twice (S1 and S2) by exposing the slices to a depolarizing buffer containing high concentration of KCl (25 mM for hippocampus; 20 mM for striatum). CPO or WIN 55,212-2 was added 20 min before the second pulse of potassium and the peak ratio of S2/S1 was used to evaluate effects on ACh release. An asterisk indicates a significant difference compared to respective controls while a pound sign indicates a significant difference in release between tissues from wild type and CB1 knockout mice. Data represent values from three independent assays.