Fig. 3.

NF myocardium (n = 2) was treated with 0U, 0.5U or 5U PP2A for 40 min at RT. Subsequently, proteins were separated by 1D gel electrophoresis (a). Phosphorylation signals of all proteins on ProQ Diamond-stained gels were divided by their SYPRO signals to correct for minor differences in loading. PKA-dependent bis-phosphorylation of cTnI at Ser23/24 was measured by Western blot analysis and normalized to α-actinin (b). cTnT troponin T, MLC-2 myosin light chain 2, MHC myosin heavy chain, MLC-1 myosin light chain 1, MW molecular weight marker