Fig. 4.
Myofilament proteins from NF and failing (ICM; DCM) hearts were separated by 1D gel electrophoresis (a). Staining differences between gels were corrected with phosphorylated ovalbumin of the peppermint marker (PM). Phosphorylation signals of all proteins on ProQ Diamond-stained gels were divided by the SYPRO signals of myosin binding protein C (cMyBP-C) to correct for minor differences in loading (b). PKA-dependent bis-phosphorylation of cTnI at Ser23/24 was measured by Western blot analysis and normalized to actin (c). cTnT troponin T, MLC-2 myosin light chain 2, MW molecular weight marker. *P < 0.05 vs. NF (post-hoc Bonferroni analysis)