Table 2.
Gene-specific forward and reverse RT-qPCR primers for genes associated with the dopaminergic system.
| Gene Name | Tyrosine Hydroxylase (Th) | Monoamine oxidase A (Maoa) |
|---|---|---|
| Forward Primer 5’ – 3’ | CCCAAGGGCTTCAGAAGAG | AGGTGGCTCTGGCCAAATAAGTGA |
| Reverse Primer 5’ – 3’ | GGGCATCCTCGATGAGACT | ACCGGTGGGATGGCACTAATTACA |
| Accession Number | NM_009377 | NM_173740 |
| Amplicon Length (Base Pairs) | 61 | 65 |
| Product Melting Temperature (°C) | 82.5 | 87.5 |
Primers were designed with either Roche primer database or Integrated DNA Technologies (IDT) Primer QuestSM. Oligo primers were analyzed with IDT Oligo Analyzer 3.1 for melting temperature, GC content and secondary structure.