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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2011 Jun 12;256(3):258–267. doi: 10.1016/j.taap.2011.05.021

Table 2.

Gene-specific forward and reverse RT-qPCR primers for genes associated with the dopaminergic system.

Gene Name Tyrosine Hydroxylase (Th) Monoamine oxidase A (Maoa)
Forward Primer 5’ – 3’ CCCAAGGGCTTCAGAAGAG AGGTGGCTCTGGCCAAATAAGTGA
Reverse Primer 5’ – 3’ GGGCATCCTCGATGAGACT ACCGGTGGGATGGCACTAATTACA
Accession Number NM_009377 NM_173740
Amplicon Length (Base Pairs) 61 65
Product Melting Temperature (°C) 82.5 87.5

Primers were designed with either Roche primer database or Integrated DNA Technologies (IDT) Primer QuestSM. Oligo primers were analyzed with IDT Oligo Analyzer 3.1 for melting temperature, GC content and secondary structure.