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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cell Signal. 2011 Aug 18;24(1):71–76. doi: 10.1016/j.cellsig.2011.07.018

Fig. 3.

Fig. 3

AS160 modulates adiponectin secretion. (A) Western blot detection of adiponectin secretion from adipocytes transfected with control vector (Ctrl) or indicated AS160 vectors. Differentiating 3T3-L1 adipocytes were electroporated with indicated vector on day 6. After two days, mature adipocytes were cultured in serum-free medium with or without 100nM Insulin for 6 h. Adiponectin profile was determined by western blotting in non-reducing condition. Wt: wild type; 4P: constitutive active form of AS160. Commasie staining shows loading control. (B) ELISA detection of secreted adiponectin from transfected adipocytes was examined by ELISA. All values represent the means ± SD., n=3; *: P < 0.05 compared to control treatment.