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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cell Signal. 2011 Sep 22;24(1):309–315. doi: 10.1016/j.cellsig.2011.09.015

Figure 1.

Figure 1

Y2H mapping of interacting regions of Protor-2 and TTP. The indicated deletion mutants of Gal4DBD-TTP or Gal4AD-Protor-2 were cotransfected into AH109 cells and plated onto selective media in the absence of histidine to determine whether an interaction between the proteins was detectable via activation of the HIS3 reporter (+++, positive growth; ++, moderate growth; −, no growth). Colonies which grew were assayed for β-gal activity as described (XX).