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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Cell Signal. 2011 Sep 22;24(1):309–315. doi: 10.1016/j.cellsig.2011.09.015

Figure 3.

Figure 3

Overexpression of Protor-2 promotes TTP-associated mRNA turnover. A. Expression of myc-tagged Protor-2 in Jurkat cells. Lane 1, JurkatPuro control transfectants, Lane 2, JurkatProtor2 overexpression clone. Western blot was probed with a-myc epotope antibodies (4E11) and actin. B. Relative mRNA turnover and half-lives (t1/2) of the indicated transcripts from JurkatPuro (shaded circles) or JurkatProtor2 (shaded squares) as determined by ActD-mRNA half-life experiments followed by real time qRT-PCR of the indicated transcripts. C. Relative mRNA turnover of the indicated mRNAs in JurkatZeo (open circles) versus TSCsiJurkat (shaded triangles) cells following tet-induced suppression of TSC1/TSC2.