Inhibition of Protor-2 expression reduces TTP association with stress granules and P-bodies. HeLa cells were treated with control, non-targeting or Protor-2-targeting siRNA and treated with FCCP (1 µM) to induce SG and P-bodies formation. Subcellular localization of TTP and stress granule (TIA-1) and P-body (Dcp1) markers (right panels, red, as indicated) were determined by dual label immunofluorescence using CARP-3, an affinity-purified antibody reactive with TTP (left panels, green). Arrows point out SGs and P-bodies. Bar size is 10 µm.