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Human recombinant 5-LOX converts both 18R and 18S-HEPE to direct precursors of RvE2.
A) Reverse-phase and chiral separation of 18S- and 18R-resolvin E2 after 5-LOX incubation and reduction. Diastereomeric mixture of RvE2 was isolated by conventional RP-HPLC separation and further resolved by chiral HPLC.
B) Tandem mass spectra of 18R-RvE2, 18S-RvE2 and C) their corresponding MS-MS assignments (see insets).
