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Negative-ion ESI-MS of total lipid extracts from E. coli lacking pgsA prepared with CHCl3 or CH2Cl2. The lipids in Panel A were extracted using a standard Bligh-Dyer (2:2:1.8, CHCl3/CH3OH/PBS, v/v/v). The lipids in Panel B were extracted using a Bligh-Dyer extraction in which methylene chloride replaced the chloroform (2:2:1.8, CH2Cl2/CH3OH/PBS, v/v/v).
