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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Biochim Biophys Acta. 2011 May 27;1811(11):946–960. doi: 10.1016/j.bbalip.2011.05.006

Fig. 4.

Fig. 4

(A) Reactive DESI mass spectrum (background subtracted) of rat brain tissue using as solvent ACN:H2O:DMF (8:3:1) doped with 65 ppm BA, showing derivatized cholesterol (S/N ≥ 100) and various phospholipids. (B) Normal DESI mass spectrum (background subtracted) of rat brain tissue using ACN:H2O:DMF (8:3:1) as spray solvent without adding the BA reagent. Note that some of the additional peaks seen in the mass range m/z 850 to 1000 of the reactive DESI (A) compared to the normal DESI spectrum (B) are reaction products of PCs with BA and water. (C) Mechanism of in situ reaction between BA cation and the hydroxyl group of an alcohol. Figure adapted from reference 63.