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. Author manuscript; available in PMC: 2012 Nov 1.

Published in final edited form as: Plast Reconstr Surg. 2011 Nov;128(5):438e–450e. doi: 10.1097/PRS.0b013e31822b7352

Fig. 5 — The effect of fasudil on alpha smooth muscle actin expression (A) Western blot analysis of 21d tissue for alpha smooth muscle actin expression (αSMA) and beta tubulin (β-tubulin) (B) Primary dermal fibroblasts were incubated with 5ng/ml TGF-β for 4d to differentiate into myofibroblasts in the presence of 12.5µM or 25µM fasudil and αSMA expression measured by intracellular flow cytometry. Data represents mean fluorescence intensity of 3 cell lines, mean ± SEM.

Fig. 5 — The effect of fasudil on alpha smooth muscle actin expression (A) Western blot analysis of 21d tissue for alpha smooth muscle actin expression (αSMA) and beta tubulin (β-tubulin) (B) Primary dermal fibroblasts were incubated with 5ng/ml TGF-β for 4d to differentiate into myofibroblasts in the presence of 12.5µM or 25µM fasudil and αSMA expression measured by intracellular flow cytometry. Data represents mean fluorescence intensity of 3 cell lines, mean ± SEM.