Skip to main content
. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: Microb Pathog. 2011 Aug 16;51(6):466–470. doi: 10.1016/j.micpath.2011.07.009

FIG. 2. Basal Stx2 protein levels.

FIG. 2

(A): Representative western blot measuring Stx2 levels among 9 strains encompassing 5 O157:H7 clades using anti-Stx2 mAbs and visualized with enhanced chemioluminescence. The location of Stx2 is indicated (inset right); (B): Graphical representation of fold-change in Stx2 levels relative to strain Sakai for strains in panel A. Bars represent mean Stx2 levels as determined using Image J. Error bars indicate standard deviation (N=3). Bars which differ in the number of asterisks, differ significantly by Tukey’s HSD following a significant F-test (P<0.05). Strain TW09178 belongs to clade 7, and is included as a stx2-stx2c+ control for cross reactivity of anti-Stx2 mAb with Stx2c.