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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: J Neurosci Methods. 2010 Nov 11;194(2):287–296. doi: 10.1016/j.jneumeth.2010.11.001

Fig. 2. Analysis of microglial population before and after magnetic separation.

Fig. 2

The proportion of microglia was determined quantitatively by flow cytometry with direct CD11b-PE labeling and also confirmed by iba1/GFAP/Hoechst immunofluorescence. Before separation, the mixed glial population contained around 26% CD11b+ microglia (B,A). After magnetic separation, the positive fraction contained more than 97% pure microglia (D,C) while the negative fraction had less than 2% microglia with abundant GFAP-positive cells (F,E). Results from one representative separation are shown. Data for multiple separations is summarized in Table1.