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. Author manuscript; available in PMC: 2012 Jan 15.
Published in final edited form as: J Neurosci Methods. 2010 Nov 11;194(2):287–296. doi: 10.1016/j.jneumeth.2010.11.001

Fig. 3. Comparison of microglial cell purity obtained by differential adherence and magnetic separation.

Fig. 3

Triple labeling immunofluorescence was with iba1/GFAP/Hoechst was used to compare microglial purity obtained by magnetic separation (A,B,C) and differential adherence (D,E,F). As shown above both methods had comparable purities with greater than 97% iba1-positive microglia. The number of Hoechst co-localized iba1-positive microglia and GFAP-positive astrocytes were also counted to quantitatively estimate the purity levels (G)