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Validation of protein-protein interactions by co-immunoprecipitation experiments. The Odin protein complex was harvested by immunoprecipitation with anti-FLAG antibodies. The cell lysates and corresponding immunoprecipitates were resolved by SDS-PAGE and probed with antibodies against talin 2 (A), SH3KBP1 (B), CD2AP (C), ARHGAP10 (D), mortalin (E), 14-3-3ε (F), ζ (G) and γ (H).
