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. 2011 Feb 2;32(21):2713–2722. doi: 10.1093/eurheartj/ehq519

Figure 7.

Figure 7

Type I collagenase activity is inhibited in human aortic fibrous caps by the fibroblast activation protein-blocking antibody A246. (A) Movat staining of a fibrous cap in human aortic plaque. The region of interest (black box) is shown at higher magnification in an adjacent section stained for fibroblast activation protein (red), type I collagen (green), and overlay (DAPI = blue; bar = 150 µm). (B) Confocal images of in situ zymography show fibroblast activation protein (red) and cleaved DQ type I collagen (green) in fibrous caps shown by Movat (A) of aortic plaque treated with a control IgG antibody or neutralizing antibody A246 (bar = 10 μm). (C) The graph reveals a significant reduction of cleaved type I collagen colocalized with fibroblast activation protein expression by in situ zymography (n = 10/group). (D) Type I collagenase activity of recombinant human fibroblast activation protein and the neutralizing capacity of A246 was demonstrated by incubation of native human type I collagen with recombinant human fibroblast activation protein in the presence of inhibiting or isotype control antibodies (lanes: 1, rhuFAP; 2, collagen; 3, rhuFAP + collagen; 4, rhuFAP + collagen + A246; 5, rhuFAP + collagen + isotype control antibody; 6, molecular weight marker).