Table 2.
Spot | Gel pI/MM | Identity match | Accession no. | MS Scorea | Maximum sequence coverage (%) | Matched fragments | MS/MS scoreb | Maximum sequence coverage (%) | Matched fragments |
---|---|---|---|---|---|---|---|---|---|
A | 5.9/40 | Haptoglobin | gi|3337390 | 71 | 39 | 10/47 | 394 | 22 | 6/59 |
B | 6.2/40 | Haptoglobin | gi|3337390 | 69 | 42 | 11/51 | 338 | 25 | 7/65 |
C | 6.3/40 | Haptoglobin | gi|3337390 | 98 | 39 | 10/51 | 355 | 22 | 6/66 |
D | 6.4/40 | Haptoglobin | gi|3337390 | 65 | 29 | 9/41 | 124 | 17 | 4/53 |
E | 6.5/40 | Haptoglobin | gi|3337390 | 75 | 33 | 9/48 | 97 | 11 | 3/58 |
F | 5.7/28 | Apolipoprotein A-1 | gi|90108664 | 153 | 53 | 16/39 | 294 | 26 | 6/53 |
MM, Molecular mass.
A minimum MS score greater than 64 represents a statistically significant match (P < 0.05).
A minimum of two significant MS/MS peptide fragment matches was required to assign an identification for a spot.