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Sequential comparison of extended focus images varying in number of focal planes, with a Multilayer stack image for comparison. With extended focus, intranuclear digital artifacts become more prominent with more focal planes. In all cases, sharp detail is significantly less than with multilayer stack method. (a) Multilayer stack image for comparison, (b) extended focus using 10 focal planes, (c) extended focus using 13 focal planes, (d) extended focus using 16 focal planes
