Figure 1.

Enhanced NF-κB activity in therapy-induced senescent primary lymphoma cells. (A) GSEAs of NF-κB targets (left) and the subset of cytokines and their modulators therein (right) in the GEP of ADR senescent versus untreated (ut) Eμ-myc control;bcl2 lymphomas (n = 12 matched pairs). (B) DNA-binding activity of the indicated NF-κB subunits in lymphomas as in A (n ≥ 3 matched pairs per subunit). (C) Immunofluorescence of the NF-κB subunit p65 in cytospin preparations of lymphoma cells as in A (representative example of three cases analyzed; bars, 50 μm). (D) Immunoblot analyses of the indicated proteins in cell lysates from four matched lymphoma pairs as in A with H3K9me3 as a senescence marker (Reimann et al. 2010) and α-Tubulin as a loading control. (E) RQ-PCR analyses of the indicated nonsecreted (left) and secreted (right) transcripts in lymphomas as in A (n = 5 samples each). Shown are relative expression levels, normalized to an internal control, that are comparable throughout all data sets. (Note the log-scaled presentation in the cytokine panel. All secreted NF-κB targets presented here reflect SASP, while Igfbp7 is a SASP factor, but not a bona fide NF-κB target.) All histogram bars indicate mean values ± standard deviation (SDEV).