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. 2011 Oct 15;25(20):2187–2197. doi: 10.1101/gad.16974811

Figure 3.

Figure 3.

Foxc2 and Integrin-α9 are expressed in the lymphovenous valves but are absent in Prox1 heterozygous embryos. E13.5 wild-type (A,C) and Prox1+/GFPCre (B,D) embryos were frontally sectioned and immunostained for either Prox1 and Foxc2 (A,B) or Prox1 and Integrin-α9 (C,D). (A) In wild-type embryos, Foxc2 is specifically expressed on the outer layer of the valve's leaflets, which are continuous with the walls of the veins (arrow). (B) In sections from an identical region in Prox1+/GFPCre embryos, Foxc2 is not expressed on the walls of the veins (arrow). Integrin-α9 is strongly expressed in the valves of control embryos (C, arrow) but is absent in Prox1+/GFPCre littermates (D, arrow). The head is oriented toward the right, the heart is oriented toward the left, and the thymus is oriented toward the bottom in both panels. (IJV) Internal jugular vein; (SCV) subclavian vein; (LS) lymph sac. Bar, 50 μm.