Table 2.

Cutoff score value for protein: 71 (score is −10∗Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 71 are significant (P < 0.05)). The software used for peak-picking was 4000 Series Explorer (TM) RAC Software, version 3.5.3 (Applied Biosystems/MDS SCIEX, Concord, Ontario, Canada). Parameters and thresholds used for peak-picking: (a) intensity or S/N threshold: S/N = 10, local noise window width (m/z) = 250, min peak width at full width half max (bins) = 2.9; (b) means of calibrating each spectrum: internal calibration with peptides from trypsin autolysis (M + H⁺ = 842.509, M + H⁺ = 2211.104); (c) resolution: 12000 for the mass 842.51 and 18000 for the mass 2211. Search parameters: (a) MASCOT (Matrix Science, London, UK) software, VERSION 2.0; (b) enzyme specificity: trypsin; (c) missed cleavages permitted: 1; (d) fixed modification (s): carbamidomethyl (C); (e) variable modifications: oxidation (M), Phospho (ST), Phospho (Y); (f) mass tolerance for precursor ions: ±100 ppm; (g) mass tolerance for fragment ions: ±0.2 Da; (h) name of database searched and release version: NCBInr 20080628 (6655203 sequences); (i) species restriction: Mammalia (mammals) (689751 sequences); (j) acceptance criteria: cut-off score value for protein: 71 (score is -10∗Log(P), where P is the probability that the observed match is a random event. Protein scores greater than 71 are significant (P < 0.05)).

Spot No	protein score	protein score 1%	Coverage %	Number of peptides identified
P1′	170	100	60	13
P2′	149	100	50	12
P1	282	100	90	24
P2	439	100	95	24
P3	288	100	64	15
P4	237	100	55	12