Table 3.
Identification of GRp58 protein in the gastric mucosa of the rat. Rat gastric proteins were separated by 2-DE and identified by means of MALDI-TOF. The protein identified represents the spots shown in Figures 1(a) and 1(b): protein disulfide-isomerase A3 precursor (Disulfide isomerase ER-60; ERp60; 58 kDa microsomal protein; p58; ERp57; HIP-70; Q-2; 35 petides), the spots labelled as P1′ and P2′ are the same spots as P1 and P2 but in different gels.
| Protein MW: 57043.9 Protein PI: 5.88 Accession No: gi|1352384| | ||||||
|---|---|---|---|---|---|---|
| Observed Mr (expt) | Mr (calc) | ± da | ± ppm | Start-end | Sequence | Spot number |
|
| ||||||
| 823.4785 | 823.4559 | 0.0226 | 27 | 190–196 | IVAYTEK | p1′,p1 |
| 866.4574 | 866.4617 | −0.0043 | 5 | 387–393 | YKELGEK | p1′,p1 |
| 877.4915 | 877.489275 | 0.0025 | 3 | 268–275 | LNFAVASR | p1′,p1 |
| 995.5628 | 995.5632 | −0.0004 | 0 | 102–111 | QAGPASVPLR | p1′,p1 |
| 997.5101 | 997.459 | −0.0511 | −51 | 153–161 | DASVVGFFR | p2 |
| 1084.5674 | 1084.5688 | 0.0014 | 1 | 95–104 | YGVSGYPTLK | p2′,p1,p2,P3 |
| 1123.6582 | 1123.6589 | 0.0007 | 1 | 101–111 | KQAGPASVPLR | p1′,p1 |
| 1125.547 | 1125.5436 | 0.0034 | 3 | 243–251 | NTKGSNYWR | p1 |
| 1172.5404 | 1172.4702 | −0.0702 | −60 | 336–344 | FVMQEEFSR | p1,p2 |
| 1179.5865 | 1179.6239 | 0.0374 | 32 | 174–183 | AASNLRDNYR | p2′,p1,p2 |
| 1188.5354 | 1188.5305 | −0.0049 | −4 | 344–336 | FVMQEEFSR | p2′,p1,p2,P3 |
| 1191.6005 | 1191.6084 | 0.0079 | 7 | 63–73 | LAPEYEAAATR | p1′,p2′,p1,p2,P3,P4 |
| 1236.5127 | 1236.5029 | −0.0098 | −8 | 108–119 | DGEEAGAYDGPR | p2′,p1,p2,P3 |
| 1244.6633 | 1244.6276 | −0.0357 | −29 | 184–194 | FAHTNVESLVK | p1,P3 |
| 1341.6837 | 1341.683 | −0.0007 | −1 | 449–460 | GFPTIYFSPANK | p2′,p1,p2,P3,P4 |
| 1347.7043 | 1347.7015 | 0.0028 | 2 | 33–44 | RLAPEYEAAATR | p1 |
| 1373.6736 | 1373.5839 | −0.0897 | −65 | 352–362 | FLQEYFDGNLK | p2 |
| 1394.6587 | 1394.5729 | −0.0858 | −62 | 162–173 | DLFSDGHSEFLK | p1,p2 |
| 1396.6954 | 1396.6727 | −0.0227 | −16 | 367–379 | SEPIPETNEGPVK | p2′,p1,p2,P3,P4 |
| 1397.5784 | 1397.7063 | 0.1279 | 92 | 83–94 | VDCTANTNTCNK | p1′,p2′,p2,P3,P4 |
| 1397.7059 | 1397.7063 | 0.0004 | 0 | 472–482 | ELNDFISYLQR | p1′,p2′,p1,P3,P4 |
| 1469.7787 | 1469.691 | −0.0877 | −60 | 449–461 | GFPTIYFSPANKK | p1,p2,P4 |
| 1472.6838 | 1472.6184 | −0.0654 | −44 | 336–347 | FVMQEEFSRDGK | p2 |
| 1488.6787 | 1488.6698 | −0.0089 | −6 | 336–347 | FVMQEEFSRDGK | P3 |
| 1529.7747 | 1529.775 | 0.0003 | 0 | 352–363 | FLQEYFDGNLKR | p1′,p2′,p1,p2,P3,P4 |
| 1593.8483 | 1593.8551 | 0.0068 | 4 | 483–496 | EATNPPIIQEEKPK | p2′,p1,p2,P3,P4 |
| 1607.7476 | 1607.764 | 0.0164 | 10 | 259–271 | DLLTAYYDVDYEK | p1′,p2′,p2,P3,P4 |
| 1636.7523 | 1636.6552 | −0.0971 | −59 | 434–448 | MDATANDVPSPYEVK | p1,p2,P4 |
| 1652.7472 | 1652.6615 | −0.0857 | −52 | 434–448 | MDATANDVPSPYEVK | p2 |
| 1652.7662 | 1652.7704 | 0.0042 | 3 | 105–119 | IFRDGEEAGAYDGPR | p1′,p2′,p1,p2,P3,P4 |
| 1744.8864 | 1744.7698 | −0.1166 | −67 | 131–146 | QAGPASVPLRTEDEFK | p2 |
| 1746.9286 | 1746.8311 | −0.0975 | −56 | 289–304 | TFLDAGHKLNFAVASR | p1′ |
| 1800.9377 | 1800.9132 | −0.0245 | −14 | 364–379 | YLKSEPIPETNEGPVK | p1′,p2′,p1,p2,P3,P4 |
| 1950.9331 | 1950.7816 | −0.1515 | −78 | 259–274 | DLLTAYYDVDYEKNTK | p2 |
| 2463.1279 | 2462.9673 | −0.1606 | −65 | 83–104 | VDCTANTNTCNKYGVSGYPTLK | p2 |