Figure 3.

FACS analyzed the CD3⁺CD8⁻IL-17⁺ cell ratio in RA patient The PBMCs were isolated by standard Ficoll-Hypaque density centrifugation. The cells were stained by anti-CD3-PE-cy5, anti-CD8-FITC, and anti-IL-17-PE. (a) First figure presented CD3⁺CD8⁻T cells were considered CD4⁺T cells in region RL, and the other three were presented healthy controls. (b) Representative IL-17 expression in CD3⁺CD8⁻T subsets from RA patients in inactive phase. (c) Representative IL-17 expression in CD3⁺CD8⁻T subsets from RA patients in active phase. (d) The results were shown as means ± SD. *P < 0.05 compared with the control group.